PDBe 1sx2

X-ray diffraction
1.06Å resolution

Use of a Halide Binding Site to Bypass the 1000-atom Limit to Structure Determination by Direct Methods

Source organism: Escherichia virus T4
Primary publication:
Use of an ion-binding site to bypass the 1000-atom limit to structure determination by direct methods.
Acta Crystallogr. D Biol. Crystallogr. 60 1726-37 (2004)
PMID: 15388918

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.59 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P3221
Unit cell:
a: 60.199Å b: 60.199Å c: 95.242Å
α: 90° β: 90° γ: 120°
R R work R free
0.125 0.125 0.143
Expression system: Escherichia coli