1srs

X-ray diffraction
3.2Å resolution

SERUM RESPONSE FACTOR (SRF) CORE COMPLEXED WITH SPECIFIC SRE DNA

Released:
Source organism: Homo sapiens
Primary publication:
Structure of serum response factor core bound to DNA.
Nature 376 490-8 (1995)
PMID: 7637780

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Serum response factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11831 (Residues: 132-223; Coverage: 18%)
Gene name: SRF
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
DNA (5'-D(*CP*CP*(5IU)P*TP*CP*CP*TP*AP*AP*TP*TP*AP*GP*GP*CP*CP*AP*TP*G)-3') Chain: W
Molecule details ›
Chain: W
Length: 19 nucleotides
Theoretical weight: 5.87 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*CP*AP*TP*GP*GP*CP*CP*TP*AP*AP*TP*TP*AP*GP*GP*A P*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.85 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 106.84Å b: 106.84Å c: 76.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 not available
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided