1smx

X-ray diffraction
1.8Å resolution

Crystal structure of the S1 domain of RNase E from E. coli (native)

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease E Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21513 (Residues: 35-125; Coverage: 9%)
Gene names: JW1071, ams, b1084, hmp1, rne
Sequence domains: S1 RNA binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 70.378Å b: 70.378Å c: 87.917Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.231
Expression system: Escherichia coli BL21