PDBe 1slw

X-ray diffraction
2Å resolution

RAT ANIONIC N143H, E151H TRYPSIN COMPLEXED TO A86H ECOTIN; NICKEL-BOUND

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ecotin Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23827 (Residues: 21-162; Coverage: 100%)
Gene names: JW2197, b2209, eco, eti
Sequence domains: Ecotin
Structure domains: Immunoglobulin-like
Anionic trypsin-2 Chain: B
Molecule details ›
Chain: B
Length: 223 amino acids
Theoretical weight: 23.86 KDa
Source organism: Rattus norvegicus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00763 (Residues: 24-246; Coverage: 97%)
Gene names: Prss2, Try2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 86.38Å b: 56.23Å c: 81.23Å
α: 90° β: 92.81° γ: 90°
R-values:
R R work R free
0.179 0.179 0.253
Expression systems:
  • Escherichia coli
  • Saccharomyces cerevisiae