1slj

Solution NMR

Solution structure of the S1 domain of RNase E from E. coli

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease E Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 10.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21513 (Residues: 35-125; Coverage: 9%)
Gene names: JW1071, ams, b1084, hmp1, rne
Sequence domains: S1 RNA binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Chemical shifts: BMR6122  
Expression system: Escherichia coli BL21