PDBe 1shy

X-ray diffraction
3.22Å resolution

The Crystal Structure of HGF beta-chain in Complex with the Sema Domain of the Met Receptor.

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hepatocyte growth factor beta chain Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 26.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14210 (Residues: 495-728; Coverage: 34%)
Gene names: HGF, HPTA
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hepatocyte growth factor receptor Chain: B
Molecule details ›
Chain: B
Length: 551 amino acids
Theoretical weight: 62.54 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08581 (Residues: 25-567; Coverage: 40%)
Gene name: MET
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21212
Unit cell:
a: 137.052Å b: 186.352Å c: 66.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.27
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda