› 1sgk

X-ray diffraction
2.3Å resolution

NUCLEOTIDE-FREE DIPHTHERIA TOXIN

Released:
DOI: 10.2210/pdb1sgk/pdb
PDB entry 1sgk coloured by chain, front view.
PDB entry 1sgk coloured by chain, side view.
PDB entry 1sgk coloured by chain, top view.
Source organism: Corynephage beta
Primary publication:
Crystal structure of nucleotide-free diphtheria toxin.
Bell CE, Eisenberg D
Biochemistry 36 481-8 (1997)
PMID: 9012663

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132743 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diphtheria toxin Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 58.41 KDa
Source organism: Corynephage beta
UniProt:
  • Canonical: P00588 (Residues: 33-567; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 105.2Å b: 91.2Å c: 65.3Å
α: 90° β: 94.2° γ: 90°
R-values:
R R work R free
0.182 0.182 0.282

Quick links

Citations

5 review citations

Biology and molecular epidemiology of diphtheria toxin and the tox gene.
Holmes RK. (2000)
4 more

6 mentions without citation

Obstructing toxin pathways by targeted pore blockage.
Nestorovich et al. (2012)
5 more