PDBe 1s5e

X-ray diffraction
1.9Å resolution

Cholera holotoxin, Crystal form 1

Released:

Function and Biology Details

Reaction catalysed:
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cholera enterotoxin subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 27.23 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01555 (Residues: 19-258; Coverage: 100%)
Gene names: VC_1457, ctxA, toxA
Structure domains: Heat-Labile Enterotoxin, subunit A
Cholera enterotoxin subunit B Chains: D, E, F, G, H, J, K, L, M, N
Molecule details ›
Chains: D, E, F, G, H, J, K, L, M, N
Length: 103 amino acids
Theoretical weight: 11.62 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01556 (Residues: 22-124; Coverage: 100%)
Gene names: VC_1456, ctxB, toxB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 59.931Å b: 108.23Å c: 122.976Å
α: 90° β: 95.89° γ: 90°
R-values:
R R work R free
0.169 0.167 0.205
Expression system: Escherichia coli BL21(DE3)