PDBe 1rrq

X-ray diffraction
2.22Å resolution

MutY adenine glycosylase in complex with DNA containing an A:oxoG pair

Released:

Function and Biology Details

Reaction catalysed:
Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Adenine DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 42.1 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P83847 (Residues: 1-365; Coverage: 100%)
Gene name: mutY
Sequence domains:
Structure domains:
5'-D(AP*AP*GP*AP*CP*(8OG)P*TP*GP*GP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.42 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(*TP*GP*TP*CP*CP*AP*AP*GP*TP*CP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.32 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, RIGAKU
Spacegroup: P212121
Unit cell:
a: 37.763Å b: 85.699Å c: 142.088Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.262
Expression systems:
  • Escherichia coli
  • Not provided