PDBe 1rio

X-ray diffraction
2.3Å resolution

Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
RNA polymerase sigma factor SigA Chain: H
Molecule details ›
Chain: H
Length: 73 amino acids
Theoretical weight: 8.72 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9EZJ8 (Residues: 366-438; Coverage: 17%)
Gene name: sigA
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Repressor protein cI Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.15 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03034 (Residues: 1-92; Coverage: 39%)
Gene names: cI, lambdap88
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
27-MER Chain: U
Molecule details ›
Chain: U
Length: 27 nucleotides
Theoretical weight: 8.29 KDa
27-MER Chain: T
Molecule details ›
Chain: T
Length: 27 nucleotides
Theoretical weight: 8.3 KDa

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P21
Unit cell:
a: 47.256Å b: 71.269Å c: 77.199Å
α: 90° β: 91.34° γ: 90°
R-values:
R R work R free
0.218 0.216 0.254
Expression system: Escherichia coli BL21(DE3)