› 1rge

X-ray diffraction
1.15Å resolution

HYDROLASE, GUANYLORIBONUCLEASE

Released:
DOI: 10.2210/pdb1rge/pdb
PDB entry 1rge coloured by chain, front view.
PDB entry 1rge coloured by chain, side view.
PDB entry 1rge coloured by chain, top view.
Source organism: Kitasatospora aureofaciens
Primary publication:
Ribonuclease from Streptomyces aureofaciens at atomic resolution.
Sevcik J, Dauter Z, Lamzin VS, Wilson KS
Acta Crystallogr D Biol Crystallogr 52 327-44 (1996)
PMID: 15299705

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease Sa Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.58 KDa
Source organism: Kitasatospora aureofaciens
UniProt:
  • Canonical: P05798 (Residues: 1-96; Coverage: 100%)
Gene name: rnaSA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand 2GP 1 x 2GP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P212121
Unit cell:
a: 64.73Å b: 78.56Å c: 38.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.109 not available not available

Quick links

Citations

2 review citations

New applications of simulated annealing in X-ray crystallography and solution NMR.
Brünger et al. (1997)
1 more

22 mentions without citation

Structural analysis of hypothetical proteins from Helicobacter pylori: an approach to estimate functions of unknown or hypothetical proteins.
Park et al. (2012)
21 more