X-ray diffraction
3.5Å resolution

Structurally Distinct Recognition Motifs in Lymphotoxin-B Receptor and CD40 for TRAF-mediated Signaling


Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TNF receptor-associated factor 3 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q13114 (Residues: 377-568; Coverage: 34%)
Gene names: CAP1, CRAF1, TRAF3
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Tumor necrosis factor receptor superfamily member 3 Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.52 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P36941 (Residues: 385-408; Coverage: 6%)
Gene names: D12S370, LTBR, TNFCR, TNFR3, TNFRSF3

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P321
Unit cell:
a: 83.7Å b: 83.7Å c: 79.1Å
α: 90° β: 90° γ: 120°
R R work R free
0.266 0.266 0.315
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided