1r8s

X-ray diffraction
1.46Å resolution

ARF1[DELTA1-17]-GDP IN COMPLEX WITH A SEC7 DOMAIN CARRYING THE MUTATION OF THE CATALYTIC GLUTAMATE TO LYSINE

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
Non-polymer only dimer (preferred)
Non-polymer only tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor 1 Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84080 (Residues: 18-181; Coverage: 91%)
Gene name: ARF1
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Cytohesin-2 Chain: E
Molecule details ›
Chain: E
Length: 203 amino acids
Theoretical weight: 23.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99418 (Residues: 50-252; Coverage: 51%)
Gene names: ARNO, CYTH2, PSCD2, PSCD2L
Sequence domains: Sec7 domain
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P3221
Unit cell:
a: 104.018Å b: 104.018Å c: 69.277Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.159 0.17
Expression system: Escherichia coli