› 1r8s

X-ray diffraction
1.46Å resolution

ARF1[DELTA1-17]-GDP IN COMPLEX WITH A SEC7 DOMAIN CARRYING THE MUTATION OF THE CATALYTIC GLUTAMATE TO LYSINE

Released:
DOI: 10.2210/pdb1r8s/pdb
PDB 1r8s coloured by chain and viewed from the front.
PDB 1r8s coloured by chain and viewed from the side.
PDB 1r8s coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor.
Renault L, Guibert B, Cherfils J
Nature 426 525-30 (2003)
PMID: 14654833

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor 1 Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84080 (Residues: 18-181; Coverage: 91%)
Gene name: ARF1
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Cytohesin-2 Chain: E
Molecule details ›
Chain: E
Length: 203 amino acids
Theoretical weight: 23.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99418 (Residues: 50-252; Coverage: 51%)
Gene names: ARNO, CYTH2, PSCD2, PSCD2L
Sequence domains: Sec7 domain
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand GDP 1 x GDP
Ligand BME 1 x BME
Ligand FMT 3 x FMT
Ligand SO4 1 x SO4
Ligand SO3 1 x SO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P3221
Unit cell:
a: 104.018Å b: 104.018Å c: 69.277Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.159 0.17
Expression system: Escherichia coli

Quick links

Citations

40 review citations

Targeting the Small GTPase Superfamily through Their Regulatory Proteins.
Gray et al. (2020)
39 more

12 mentions without citation

Allosteric regulation of Arf GTPases and their GEFs at the membrane interface.
Nawrotek et al. (2016)
11 more