› 1r7s

X-ray diffraction
1.91Å resolution

PUTIDAREDOXIN (Fe2S2 ferredoxin), C73G mutant

Released:
DOI: 10.2210/pdb1r7s/pdb
PDB entry 1r7s coloured by chain, front view.
PDB entry 1r7s coloured by chain, side view.
PDB entry 1r7s coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Structure of C73G putidaredoxin from Pseudomonas putida.
Smith N, Mayhew M, Holden MJ, Kelly H, Robinson H, Heroux A, Vilker VL, Gallagher DT
Acta Crystallogr D Biol Crystallogr 60 816-22 (2004)
PMID: 15103126

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putidaredoxin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 106 amino acids
Theoretical weight: 11.38 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00259 (Residues: 2-107; Coverage: 99%)
Gene name: camB
Sequence domains: 2Fe-2S iron-sulfur cluster binding domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand FES 3 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: C2
Unit cell:
a: 145.076Å b: 78.218Å c: 55.739Å
α: 90° β: 111.33° γ: 90°
R-values:
R R work R free
0.188 0.188 0.211
Expression system: Escherichia coli

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Citations

3 citation in other articles

Redox-dependent structural reorganization in putidaredoxin, a vertebrate-type [2Fe-2S] ferredoxin from Pseudomonas putida.
Sevrioukova IF. (2005)
2 more

2 mentions without citation

A score of the ability of a three-dimensional protein model to retrieve its own sequence as a quantitative measure of its quality and appropriateness.
Martínez-Castilla et al. (2010)
1 more