› 1qiy

X-ray diffraction
2.3Å resolution

HUMAN INSULIN HEXAMERS WITH CHAIN B HIS MUTATED TO TYR COMPLEXED WITH PHENOL

Released:
DOI: 10.2210/pdb1qiy/pdb
PDB 1qiy coloured by chain and viewed from the front.
PDB 1qiy coloured by chain and viewed from the side.
PDB 1qiy coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural consequences of the B5 histidine --> tyrosine mutation in human insulin characterized by X-ray crystallography and conformational analysis.
Tang L, Whittingham JL, Verma CS, Caves LS, Dodson GG
Biochemistry 38 12041-51 (1999)
PMID: 10508408

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 30 amino acids
Theoretical weight: 3.46 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS

Ligands and Environments

3 bound ligands:
Ligand IPH 6 x IPH
Ligand ZN 2 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 61.1Å b: 62.08Å c: 48.35Å
α: 90° β: 109.87° γ: 90°
R-values:
R R work R free
0.186 0.186 not available
Expression system: Saccharomyces cerevisiae

Quick links

Citations

7 citation in other articles

Intracellular retention of newly synthesized insulin in yeast is caused by endoproteolytic processing in the Golgi complex.
Zhang et al. (2001)
6 more