1qav

X-ray diffraction
1.9Å resolution

Unexpected Modes of PDZ Domain Scaffolding Revealed by Structure of NNOS-Syntrophin Complex

Released:

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-1-syntrophin Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q61234 (Residues: 75-164; Coverage: 18%)
Gene names: Snt1, Snta1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
Nitric oxide synthase, brain Chain: B
Molecule details ›
Chain: B
Length: 115 amino acids
Theoretical weight: 12.37 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29476 (Residues: 12-126; Coverage: 8%)
Gene names: Bnos, Nos1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1, ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 31.58Å b: 34.31Å c: 187.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.259
Expression system: Escherichia coli