X-ray diffraction
3.1Å resolution

Cobalamin-dependent methionine synthase (1-566) from T. maritima (Oxidized, Orthorhombic)


Function and Biology Details

Reaction catalysed:
5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine
Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
5-methyltetrahydrofolate--homocysteine methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 566 amino acids
Theoretical weight: 63.63 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9WYA5 (Residues: 1-566; Coverage: 74%)
Gene name: TM_0268
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 92.583Å b: 169.022Å c: 174.276Å
α: 90° β: 90° γ: 90°
R R work R free
0.253 0.221 0.265
Expression system: Escherichia coli BL21(DE3)