1q78

X-ray diffraction
2.8Å resolution

Crystal structure of poly(A) polymerase in complex with 3'-dATP and magnesium chloride

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A) polymerase alpha Chain: A
Molecule details ›
Chain: A
Length: 514 amino acids
Theoretical weight: 59.22 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25500 (Residues: 1-514; Coverage: 70%)
Gene names: PAP, PAPOLA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 57.52Å b: 63.81Å c: 180.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.255
Expression system: Escherichia coli BL21(DE3)