PDBe 1pyl

X-ray diffraction
1.51Å resolution

Crystal structure of Ribonuclease Sa2

Source organism: Kitasatospora aureofaciens
Primary publication:
Crystal structure reveals two alternative conformations in the active site of ribonuclease Sa2.
Acta Crystallogr. D Biol. Crystallogr. 60 1198-204 (2004)
PMID: 15213380

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 10.91 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
  • Canonical: Q53752 (Residues: 67-163; Coverage: 78%)
Gene names: B6264_17650, HS99_0012425, ribonuclease
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 84.98Å b: 34.13Å c: 72.27Å
α: 90° β: 109.5° γ: 90°
R R work R free
0.15 0.149 0.172
Expression system: Escherichia coli