PDBe 1py3

X-ray diffraction
1.8Å resolution

Crystal structure of Ribonuclease Sa2

Released:
Source organism: Kitasatospora aureofaciens
Primary publication:
Crystal structure reveals two alternative conformations in the active site of ribonuclease Sa2.
Acta Crystallogr. D Biol. Crystallogr. 60 1198-204 (2004)
PMID: 15213380

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 97 amino acids
Theoretical weight: 10.91 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53752 (Residues: 67-163; Coverage: 78%)
Gene names: B6264_17650, HS99_0012425, ribonuclease
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: C2
Unit cell:
a: 102.29Å b: 68.7Å c: 57.51Å
α: 90° β: 100.4° γ: 90°
R-values:
R R work R free
0.175 0.172 0.218
Expression system: Escherichia coli