X-ray diffraction
2.8Å resolution

Crystal Structure of TDP-epi-Vancosaminyltransferase GtfA in complexes with TDP and Vancomycin


Function and Biology Details

Reaction catalysed:
dTDP-beta-L-4-epi-vancosamine + devancoaminyl-vancomycin = dTDP + chloroorienticin B
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
dTDP-epi-vancosaminyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 42.77 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P96558 (Residues: 1-396; Coverage: 100%)
Gene name: gtfA
Sequence domains: Glycosyltransferase family 28 N-terminal domain
Structure domains: Glycogen Phosphorylase B;
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 1.15 KDa
Source organism: Amycolatopsis orientalis

Ligands and Environments

3 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P3221
Unit cell:
a: 153.17Å b: 153.17Å c: 100.64Å
α: 90° β: 90° γ: 120°
R R work R free
0.221 0.221 0.269
Expression system: Escherichia coli BL21(DE3)