1pk1

X-ray diffraction
1.8Å resolution

Hetero SAM domain structure of Ph and Scm.

Released:
Source organism: Drosophila melanogaster
Primary publication:
Structural organization of a Sex-comb-on-midleg/polyhomeotic copolymer.
J Biol Chem 280 27769-75 (2005)
PMID: 15905166

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-153946 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polyhomeotic-proximal chromatin protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 89 amino acids
Theoretical weight: 10.32 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P39769 (Residues: 1502-1577; Coverage: 5%)
Gene names: CG18412, ph-p
Sequence domains: SAM domain (Sterile alpha motif)
Structure domains: Transcription Factor, Ets-1
Polycomb protein Scm Chains: B, D
Molecule details ›
Chains: B, D
Length: 89 amino acids
Theoretical weight: 10.41 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VHA0 (Residues: 795-871; Coverage: 9%)
Gene names: CG9495, Scm
Sequence domains: SAM domain (Sterile alpha motif)
Structure domains: Transcription Factor, Ets-1

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 30.277Å b: 46.866Å c: 123.987Å
α: 90° β: 90.72° γ: 90°
R-values:
R R work R free
0.233 0.233 0.249
Expression system: Escherichia coli