1p3q

X-ray diffraction
1.7Å resolution

Mechanism of Ubiquitin Recognition by the CUE Domain of VPS9

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vacuolar protein sorting-associated protein 9 Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 54 amino acids
Theoretical weight: 6.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P54787 (Residues: 398-451; Coverage: 12%)
Gene names: VPS9, VPT9, YML097C
Sequence domains: CUE domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain
Ubiquitin Chains: U, V
Molecule details ›
Chains: U, V
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P0CH28 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 101.607Å b: 45.887Å c: 57.802Å
α: 90° β: 96.53° γ: 90°
R-values:
R R work R free
0.26 0.26 0.277
Expression system: Escherichia coli