1ou8

X-ray diffraction
1.6Å resolution

structure of an AAA+ protease delivery protein in complex with a peptide degradation tag

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Stringent starvation protein B homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.64 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45206 (Residues: 1-111; Coverage: 74%)
Gene names: HI_1440, sspB
Structure domains: SspB-like
synthetic ssrA peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.21 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 69.02Å b: 69.02Å c: 104.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.206 0.225
Expression systems:
  • Escherichia coli
  • Not provided