1or7

X-ray diffraction
2Å resolution

Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ECF RNA polymerase sigma-E factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AGB6 (Residues: 1-191; Coverage: 100%)
Gene names: JW2557, b2573, rpoE, sigE
Sequence domains:
Structure domains:
Anti-sigma-E factor RseA Chains: C, F
Molecule details ›
Chains: C, F
Length: 90 amino acids
Theoretical weight: 10.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFX7 (Residues: 1-90; Coverage: 42%)
Gene names: JW2556, b2572, mclA, rseA, yfiJ
Sequence domains: Anti sigma-E protein RseA, N-terminal domain
Structure domains: Anti sigma-E protein RseA, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: C2
Unit cell:
a: 140.049Å b: 56.901Å c: 104.981Å
α: 90° β: 130.51° γ: 90°
R-values:
R R work R free
0.199 0.197 0.231
Expression system: Escherichia coli BL21(DE3)