1ofh

X-ray diffraction
2.5Å resolution

Asymmetric complex between HslV and I-domain deleted HslU (H. influenzae)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-dependent protease ATPase subunit HslU Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 310 amino acids
Theoretical weight: 34.13 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43773 (Residues: 1-444; Coverage: 70%)
Gene names: HI_0497, hslU
Sequence domains: C-terminal, D2-small domain, of ClpB protein
Structure domains:
ATP-dependent protease subunit HslV Chains: G, H, I, L, M, N
Molecule details ›
Chains: G, H, I, L, M, N
Length: 174 amino acids
Theoretical weight: 18.9 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43772 (Residues: 2-175; Coverage: 99%)
Gene names: HI_0496, hslV
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6
Unit cell:
a: 192.22Å b: 192.22Å c: 115.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.224 0.277
Expression system: Escherichia coli BL21(DE3)