PDBe 1o5e

X-ray diffraction
1.75Å resolution

Dissecting and Designing Inhibitor Selectivity Determinants at the S1 site Using an Artificial Ala190 Protease (Ala190 uPA)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine protease hepsin non-catalytic chain Chain: L
Molecule details ›
Chain: L
Length: 114 amino acids
Theoretical weight: 12.52 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P05981 (Residues: 46-159; Coverage: 27%)
Gene names: HPN, TMPRSS1
Sequence domains: Hepsin, SRCR domain
Structure domains: SRCR-like domain
Serine protease hepsin catalytic chain Chain: H
Molecule details ›
Chain: H
Length: 255 amino acids
Theoretical weight: 27.57 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P05981 (Residues: 163-417; Coverage: 61%)
Gene names: HPN, TMPRSS1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 58.77Å b: 47.88Å c: 63.33Å
α: 90° β: 104.77° γ: 90°
R-values:
R R work R free
0.2 0.198 0.227
Expression system: Komagataella pastoris