1o3s

X-ray diffraction
3Å resolution

PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
cAMP-activated global transcriptional regulator CRP Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 9-208; Coverage: 95%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
5'-D(*AP*AP*AP*AP*AP*TP*GP*CP*GP*AP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*TP*AP*GP*AP*TP*CP*GP*CP*AP*TP*TP*TP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.55 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 78.05Å b: 78.05Å c: 142.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.26 0.308
Expression systems:
  • Escherichia coli
  • Not provided