X-ray diffraction
2.11Å resolution

Glyceraldehyde-3-Phosphate Dehydrogenase Mutant With Cys 149 Replaced By Ser Complexed With Nad+


Function and Biology Details

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, C, O, Q
Molecule details ›
Chains: A, C, O, Q
Length: 334 amino acids
Theoretical weight: 35.97 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
  • Canonical: P00362 (Residues: 2-335; Coverage: 100%)
Gene name: gap
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand NAD 4 x NAD
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: C2
Unit cell:
a: 140.48Å b: 87.909Å c: 119.925Å
α: 90° β: 119° γ: 90°
R R work R free
0.203 0.198 0.249
Expression system: Escherichia coli