› 1nkp

X-ray diffraction
1.8Å resolution

Crystal structure of Myc-Max recognizing DNA

Released:
DOI: 10.2210/pdb1nkp/pdb
PDB entry 1nkp coloured by chain, front view.
PDB entry 1nkp coloured by chain, side view.
PDB entry 1nkp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structures of Myc-Max and Mad-Max recognizing DNA. Molecular bases of regulation by proto-oncogenic transcription factors.
Nair SK, Burley SK
Cell 112 193-205 (2003)
PMID: 12553908

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114266 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Myc proto-oncogene protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 88 amino acids
Theoretical weight: 10.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01106 (Residues: 368-449; Coverage: 18%)
Gene names: BHLHE39, MYC
Sequence domains:
Structure domains: Helix-loop-helix DNA-binding domain
Protein max Chains: B, E
Molecule details ›
Chains: B, E
Length: 83 amino acids
Theoretical weight: 9.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61244 (Residues: 23-102; Coverage: 50%)
Gene names: BHLHD4, MAX
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain
5'-D(*CP*GP*AP*GP*TP*AP*GP*CP*AP*CP*GP*TP*GP*CP*TP*AP*CP*TP*C)-3' Chains: F, G, H, J
Molecule details ›
Chains: F, G, H, J
Length: 19 nucleotides
Theoretical weight: 5.81 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 39.244Å b: 45.128Å c: 86.484Å
α: 87.91° β: 84.61° γ: 71.5°
R-values:
R R work R free
0.219 0.219 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

51 review citations

MYC on the path to cancer.
Dang CV. (2012)
50 more

108 mentions without citation

An overview of the basic helix-loop-helix proteins.
Jones S. (2004)
107 more