PDBe 1nkg

X-ray diffraction
1.5Å resolution

Rhamnogalacturonan lyase from Aspergillus aculeatus

Released:

Function and Biology Details

Reaction catalysed:
Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhamnogalacturonate lyase A Chain: A
Molecule details ›
Chain: A
Length: 508 amino acids
Theoretical weight: 54.24 KDa
Source organism: Aspergillus aculeatus
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: Q00019 (Residues: 20-527; Coverage: 100%)
Gene names: RGL4, rglA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 77.009Å b: 77.009Å c: 170.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.164 0.186
Expression system: Aspergillus oryzae