1nk8

X-ray diffraction
1.9Å resolution

A BACILLUS DNA POLYMERASE I PRODUCT COMPLEX BOUND TO A GUANINE-THYMINE MISMATCH AFTER A SINGLE ROUND OF PRIMER EXTENSION, FOLLOWING INCORPORATION OF DCTP.

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52026 (Residues: 297-876; Coverage: 66%)
Gene names: pol, polA
Sequence domains: DNA polymerase family A
Structure domains:
DNA PRIMER STRAND Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
DNA TEMPLATE STRAND Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.6 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 87.723Å b: 93.579Å c: 105.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.19 0.219
Expression systems:
  • Escherichia coli
  • Not provided