PDBe 1n4m

X-ray diffraction
2.2Å resolution

Structure of Rb tumor suppressor bound to the transactivation domain of E2F-2

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero trimer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Retinoblastoma-associated protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 40.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06400 (Residues: 380-785; Coverage: 37%)
Gene name: RB1
Sequence domains: Retinoblastoma-associated protein B domain
Structure domains: Cyclin-like
Transcription factor E2F2 Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 18 amino acids
Theoretical weight: 2.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q14209 (Residues: 410-427; Coverage: 4%)
Gene name: E2F2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: P1
Unit cell:
a: 54.369Å b: 65.164Å c: 69.339Å
α: 85.55° β: 79.48° γ: 67.16°
R-values:
R R work R free
0.222 0.222 0.282
Expression systems:
  • Escherichia coli
  • Not provided