1n1h

X-ray diffraction
2.8Å resolution

Initiation complex of polymerase lambda3 from reovirus

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-directed RNA polymerase lambda-3 Chain: A
Molecule details ›
Chain: A
Length: 1267 amino acids
Theoretical weight: 142.42 KDa
Source organism: Mammalian orthoreovirus 3 Dearing
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0CK31 (Residues: 1-1267; Coverage: 100%)
Gene name: L1
Sequence domains: Reovirus RNA-dependent RNA polymerase lambda 3
5'-R(*AP*UP*UP*AP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 6 nucleotides
Theoretical weight: 1.88 KDa
Source organism: Mammalian orthoreovirus 3 Dearing
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 70.883Å b: 85.013Å c: 249.294Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.268
Expression systems:
  • Spodoptera frugiperda
  • Not provided