› 1mz8

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURES OF THE NUCLEASE DOMAIN OF COLE7/IM7 IN COMPLEX WITH A PHOSPHATE ION AND A ZINC ION

Released:
DOI: 10.2210/pdb1mz8/pdb
PDB entry 1mz8 coloured by chain, front view.
PDB entry 1mz8 coloured by chain, side view.
PDB entry 1mz8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Metal ions and phosphate binding in the H-N-H motif: crystal structures of the nuclease domain of ColE7/Im7 in complex with a phosphate ion and different divalent metal ions.
Sui MJ, Tsai LC, Hsia KC, Doudeva LG, Ku WY, Han GW, Yuan HS
Protein Sci 11 2947-57 (2002)
PMID: 12441392

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169934 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E7 immunity protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 87 amino acids
Theoretical weight: 9.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03708 (Residues: 1-87; Coverage: 100%)
Gene names: ceiE7, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E7 Chains: B, D
Molecule details ›
Chains: B, D
Length: 131 amino acids
Theoretical weight: 15.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 446-576; Coverage: 23%)
Gene names: cea, colE7
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P21212
Unit cell:
a: 119.735Å b: 62.41Å c: 74.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.23
Expression system: Escherichia coli

Quick links

Citations

29 citation in other articles

Structural and functional characterization of mitochondrial EndoG, a sugar non-specific nuclease which plays an important role during apoptosis.
Schäfer et al. (2004)
28 more

5 mentions without citation

Metal ions and phosphate binding in the H-N-H motif: crystal structures of the nuclease domain of ColE7/Im7 in complex with a phosphate ion and different divalent metal ions.
Sui et al. (2002)
4 more