› 1mwm

X-ray diffraction
2Å resolution

ParM from plasmid R1 ADP form

Released:
DOI: 10.2210/pdb1mwm/pdb
PDB entry 1mwm coloured by chain, front view.
PDB entry 1mwm coloured by chain, side view.
PDB entry 1mwm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
F-actin-like filaments formed by plasmid segregation protein ParM.
van den Ent F, Møller-Jensen J, Amos LA, Gerdes K, Löwe J
EMBO J 21 6935-43 (2002)
PMID: 12486014

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145973 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasmid segregation protein ParM Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 35.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11904 (Residues: 1-320; Coverage: 100%)
Gene names: parM, stbA
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P41
Unit cell:
a: 64.71Å b: 64.71Å c: 199.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

56 review citations

Actin structure and function.
Dominguez et al. (2011)
55 more

18 mentions without citation

The bacterial cytoskeleton.
Shih et al. (2006)
17 more