1mjp

X-ray diffraction
3.4Å resolution

METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO THE MINIMAL MET CONSENSUS OPERATOR

Released:
Source organism: Escherichia coli
Primary publication:
Direct and indirect readout in mutant Met repressor-operator complexes.
Structure 8 905-14 (2000)
PMID: 10986458

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Met repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 12.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8U6 (Residues: 2-105; Coverage: 99%)
Gene names: JW3909, b3938, metJ
Sequence domains: Met Apo-repressor, MetJ
Structure domains: MET Apo-Repressor, subunit A
CONSENSUS OPERATOR DUPLEX Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.04 KDa
Source organism: Escherichia coli
Expression system: Not provided
CONSENSUS OPERATOR DUPLEX Chain: D
Molecule details ›
Chain: D
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6222
Unit cell:
a: 118.39Å b: 118.39Å c: 83.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.309
Expression systems:
  • Escherichia coli
  • Not provided