PDBe 1mg2

X-ray diffraction
2.25Å resolution

MUTATION OF ALPHA PHE55 OF METHYLAMINE DEHYDROGENASE ALTERS THE REORGANIZATION ENERGY AND ELECTRONIC COUPLING FOR ITS ELECTRON TRANSFER REACTION WITH AMICYANIN

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Methylamine dehydrogenase heavy chain Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 390 amino acids
Theoretical weight: 42.67 KDa
Source organism: Paracoccus denitrificans
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P29894 (Residues: 28-417; Coverage: 100%)
Gene name: mauB
Sequence domains: Methylamine dehydrogenase heavy chain (MADH)
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Methylamine dehydrogenase light chain Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 131 amino acids
Theoretical weight: 14.21 KDa
Source organism: Paracoccus denitrificans
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P22619 (Residues: 58-188; Coverage: 100%)
Gene name: mauA
Sequence domains: Methylamine dehydrogenase, L chain
Structure domains: Methylamine/Aralkylamine dehydrogenase light chain
Amicyanin Chains: C, G, K, O
Molecule details ›
Chains: C, G, K, O
Length: 105 amino acids
Theoretical weight: 11.51 KDa
Source organism: Paracoccus denitrificans
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P22364 (Residues: 27-131; Coverage: 100%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Cytochrome c-L Chains: D, H, L, P
Molecule details ›
Chains: D, H, L, P
Length: 155 amino acids
Theoretical weight: 17.09 KDa
Source organism: Paracoccus denitrificans
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P29899 (Residues: 23-177; Coverage: 100%)
Gene name: moxG
Sequence domains: Cytochrome C oxidase, cbb3-type, subunit III
Structure domains: Cytochrome c-like domain

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 79.122Å b: 188.201Å c: 127.1Å
α: 90° β: 99.24° γ: 90°
R-values:
R R work R free
0.173 0.173 0.21
Expression system: Rhodobacter sphaeroides