› 1mdv

X-ray diffraction
2.3Å resolution

KEY ROLE OF PHENYLALANINE 20 IN CYTOCHROME C3: STRUCTURE, STABILITY AND FUNCTION STUDIES

Released:
DOI: 10.2210/pdb1mdv/pdb
PDB entry 1mdv coloured by chain, front view.
PDB entry 1mdv coloured by chain, side view.
PDB entry 1mdv coloured by chain, top view.
Source organism: Desulfovibrio vulgaris str. Hildenborough
Primary publication:
Key role of phenylalanine 20 in cytochrome c3: structure, stability, and function studies.
Dolla A, Arnoux P, Protasevich I, Lobachov V, Brugna M, Giudici-Orticoni MT, Haser R, Czjzek M, Makarov A, Bruschi M
Biochemistry 38 33-41 (1999)
PMID: 9890880

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-132278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 11.65 KDa
Source organism: Desulfovibrio vulgaris str. Hildenborough
Expression system: Desulfovibrio desulfuricans
UniProt:
  • Canonical: P00131 (Residues: 23-129; Coverage: 100%)
Gene name: DVU_3171
Sequence domains: Class III cytochrome C family
Structure domains: Cytochrome C3

Ligands and Environments

1 bound ligand:
Ligand HEM 8 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P4
Unit cell:
a: 97.22Å b: 97.22Å c: 36.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.296
Expression system: Desulfovibrio desulfuricans

Quick links

Citations

2 review citations

Implication for buried polar contacts and ion pairs in hyperthermostable enzymes.
Matsui et al. (2007)
1 more