1m8x

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-14 RNA

Released:
Source organism: Homo sapiens
Primary publication:
Modular recognition of RNA by a human pumilio-homology domain.
Cell 110 501-12 (2002)
PMID: 12202039

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Pumilio homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 40.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14671 (Residues: 828-1176; Coverage: 29%)
Gene names: KIAA0099, PUM1, PUMH1
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
5'-R(P*UP*UP*GP*UP*AP*UP*AP*U)-3' Chain: C
Molecule details ›
Chain: C
Length: 8 nucleotides
Theoretical weight: 2.49 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-R(P*UP*GP*UP*AP*UP*AP*U)-3' Chain: D
Molecule details ›
Chain: D
Length: 7 nucleotides
Theoretical weight: 2.18 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 262.73Å b: 37.73Å c: 82.52Å
α: 90° β: 102.95° γ: 90°
R-values:
R R work R free
0.218 0.236 0.293
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided