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X-ray diffraction
2Å resolution

Structure and Mechanism of T4 Polynucleotide Kinase

Released:
Source organism: Escherichia virus T4
Primary publication:
Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme.
EMBO J. 21 3873-80 (2002)
PMID: 12110598

Function and Biology Details

Reactions catalysed:
A deoxyribonucleoside 3'-phosphate + H(2)O = a deoxyribonucleoside + phosphate
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polynucleotide kinase Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.67 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06855 (Residues: 1-181; Coverage: 60%)
Gene name: pseT
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: C2221
Unit cell:
a: 65.3Å b: 91.8Å c: 67.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.231
Expression system: Escherichia coli BL21(DE3)