PDBe 1lws

X-ray diffraction
3.5Å resolution

Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Endonuclease PI-SceI Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 51.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Best match: P17255 (Residues: 284-737)
Gene names: CLS8, D1286, TFP1, VMA1, YDL185W
PI-SceI DNA recognition region top strand Chain: B
Molecule details ›
Chain: B
Length: 37 nucleotides
Theoretical weight: 11.6 KDa
Source organism: Synthetic construct
Expression system: Not provided
PI-SceI DNA recognition region bottom strand Chain: C
Molecule details ›
Chain: C
Length: 37 nucleotides
Theoretical weight: 11.17 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P6522
Unit cell:
a: 123Å b: 123Å c: 211.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.293 0.287 0.31
Expression systems:
  • Escherichia coli
  • Not provided