PDBe 1ltx

X-ray diffraction
2.7Å resolution

Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Geranylgeranyl transferase type-2 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 567 amino acids
Theoretical weight: 64.98 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08602 (Residues: 1-567; Coverage: 100%)
Gene names: Ggta, Rabggta
Sequence domains:
Structure domains:
Geranylgeranyl transferase type-2 subunit beta Chain: B
Molecule details ›
Chain: B
Length: 331 amino acids
Theoretical weight: 36.89 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08603 (Residues: 1-331; Coverage: 100%)
Gene names: Ggtb, Rabggtb
Sequence domains: Prenyltransferase and squalene oxidase repeat
Structure domains: Glycosyltransferase
Rab proteins geranylgeranyltransferase component A 1 Chain: R
Molecule details ›
Chain: R
Length: 650 amino acids
Theoretical weight: 72.59 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P37727 (Residues: 1-650; Coverage: 100%)
Gene names: Chm, Rep1
Sequence domains: GDP dissociation inhibitor
Structure domains:
AAAA Chain: P
Molecule details ›
Chain: P
Length: 4 amino acids
Theoretical weight: 302 Da

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 68.7Å b: 197.3Å c: 85.3Å
α: 90° β: 112.8° γ: 90°
R-values:
R R work R free
0.224 0.224 0.272
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda