1lr6

X-ray diffraction
1.9Å resolution

Crystal structure of V45Y mutant of cytochrome b5

Released:
Source organism: Bos taurus
Primary publication:
Structures of V45E and V45Y mutants and structure comparison of a variety of cytochrome b5 mutants.
Acta Crystallogr. D Biol. Crystallogr. 58 1298-306 (2002)
PMID: 12136141

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 9.54 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00171 (Residues: 8-89; Coverage: 61%)
Gene names: CYB5, CYB5A
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 69.404Å b: 40.441Å c: 39.196Å
α: 90° β: 111.7° γ: 90°
R-values:
R R work R free
0.196 0.191 0.236
Expression system: Escherichia coli