› 1loz

X-ray diffraction
1.8Å resolution

AMYLOIDOGENIC VARIANT (I56T) VARIANT OF HUMAN LYSOZYME

Released:
DOI: 10.2210/pdb1loz/pdb
PDB entry 1loz coloured by chain, front view.
PDB entry 1loz coloured by chain, side view.
PDB entry 1loz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Instability, unfolding and aggregation of human lysozyme variants underlying amyloid fibrillogenesis.
Booth DR, Sunde M, Bellotti V, Robinson CV, Hutchinson WL, Fraser PE, Hawkins PN, Dobson CM, Radford SE, Blake CC, Pepys MB
Nature 385 787-93 (1997)
PMID: 9039909

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 14.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61626 (Residues: 19-148; Coverage: 100%)
Gene names: LYZ, LZM
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 56.847Å b: 60.889Å c: 33.699Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 not available
Expression system: Spodoptera frugiperda

Quick links

Citations

102 review citations

Molecular mechanisms of amyloidosis.
Merlini et al. (2003)
101 more

3 mentions without citation

A nanobody binding to non-amyloidogenic regions of the protein human lysozyme enhances partial unfolding but inhibits amyloid fibril formation.
De Genst et al. (2013)
2 more