PDBe 1lbg

X-ray diffraction
4.8Å resolution

LACTOSE OPERON REPRESSOR BOUND TO 21-BASE PAIR SYMMETRIC OPERATOR DNA, ALPHA CARBONS ONLY

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Lactose operon repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 360 amino acids
Theoretical weight: 38.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-360; Coverage: 100%)
Gene names: JW0336, b0345, lacI
Sequence domains:
DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*T)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 21 nucleotides
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 104.3Å b: 224.4Å c: 112.1Å
α: 90° β: 95.7° γ: 90°
R-values:
R R work R free
0.26 0.26 not available
Expression systems:
  • Escherichia coli
  • Not provided