1lb2

X-ray diffraction
3.1Å resolution

Structure of the E. coli alpha C-terminal domain of RNA polymerase in complex with CAP and DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
cAMP-activated global transcriptional regulator CRP Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 2-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit alpha Chains: B, E
Molecule details ›
Chains: B, E
Length: 84 amino acids
Theoretical weight: 9.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 246-329; Coverage: 26%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Structure domains: 5' to 3' exonuclease, C-terminal subdomain
5'-D(*CP*TP*TP*TP*TP*TP*TP*CP*CP*TP*AP*AP*AP*AP*TP*GP*TP*GP*AP*T)-3' Chain: K
Molecule details ›
Chain: K
Length: 20 nucleotides
Theoretical weight: 6.09 KDa
5'-D(*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*AP*G)-3' Chain: J
Molecule details ›
Chain: J
Length: 24 nucleotides
Theoretical weight: 7.41 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6222
Unit cell:
a: 175.97Å b: 175.97Å c: 158.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.244
Expression system: Escherichia coli