PDBe 1l9z

X-ray diffraction
6.5Å resolution

Thermus aquaticus RNA Polymerase Holoenzyme/Fork-Junction Promoter DNA Complex at 6.5 A Resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
RNA POLYMERASE, ALPHA SUBUNIT Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 34.83 KDa
Source organism: Thermus aquaticus
RNA POLYMERASE, BETA SUBUNIT Chain: C
Molecule details ›
Chain: C
Length: 1118 amino acids
Theoretical weight: 124.8 KDa
Source organism: Thermus aquaticus
RNA POLYMERASE, BETA-PRIME SUBUNIT Chain: D
Molecule details ›
Chain: D
Length: 1524 amino acids
Theoretical weight: 171.19 KDa
Source organism: Thermus aquaticus
RNA POLYMERASE, OMEGA SUBUNIT Chain: E
Molecule details ›
Chain: E
Length: 99 amino acids
Theoretical weight: 11.64 KDa
Source organism: Thermus aquaticus
SIGMA FACTOR SIGA Chain: H
Molecule details ›
Chain: H
Length: 438 amino acids
Theoretical weight: 49.92 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
nontemplate DNA strand Chain: U
Molecule details ›
Chain: U
Length: 35 nucleotides
Theoretical weight: 10.8 KDa
template DNA strand Chain: T
Molecule details ›
Chain: T
Length: 30 nucleotides
Theoretical weight: 9.17 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P4322
Unit cell:
a: 180.947Å b: 180.947Å c: 523.461Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli BL21(DE3)