› 1l5i

Solution NMR

30-CONFORMER NMR ENSEMBLE OF THE N-TERMINAL, DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN FROM A GEMINIVIRUS (TOMATO YELLOW LEAF CURL VIRUS-SARDINIA)

Released:
DOI: 10.2210/pdb1l5i/pdb
PDB entry 1l5i coloured by chain, ensemble of 30 models, front view.
PDB entry 1l5i coloured by chain, ensemble of 30 models, side view.
PDB entry 1l5i coloured by chain, ensemble of 30 models, top view.
Source organism: Tomato yellow leaf curl Sardinia virus
Primary publication:
The structure of a replication initiator unites diverse aspects of nucleic acid metabolism.
Campos-Olivas R, Louis JM, Clerot D, Gronenborn B, Gronenborn AM
Proc Natl Acad Sci U S A 99 10310-5 (2002)
PMID: 12130667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication-associated protein Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 13.58 KDa
Source organism: Tomato yellow leaf curl Sardinia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P27260 (Residues: 4-121; Coverage: 33%)
Gene name: C1
Sequence domains: Geminivirus Rep catalytic domain
Structure domains: Replication Protein E1; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: TORSION ANGLE SIMULATED ANHEALING
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Plasmid rolling-circle replication: highlights of two decades of research.
Khan SA. (2005)
12 more

5 mentions without citation

Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158.
Boer et al. (2016)
4 more