PDBe 1l1m

Solution NMR

SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O1

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Lactose operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 62 amino acids
Theoretical weight: 6.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-62; Coverage: 17%)
Gene names: JW0336, b0345, lacI
Sequence domains: Bacterial regulatory proteins, lacI family
Structure domains: lambda repressor-like DNA-binding domains
5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*GP*AP*TP*AP*AP*CP*AP*AP*TP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7.14 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*AP*AP*AP*TP*TP*GP*TP*TP*AP*TP*CP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*TP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 6.97 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 67%
Refinement method: SIMULATED ANNEALING FOLLOWED BY RESTRAINED MDR
Expression systems:
  • Escherichia coli
  • Not provided